Differential immunoadsorption coupled with rate nephelometry for estimation of DNA-binding immunoglobulins.

Differential immunoadsorption coupled with rate nephelometry for estimation of DNA-binding immunoglobulins.

We describe a technique for estimating the mass of anti-DNA antibodies by immunonephelometry of serum immunoglobulins (IgG, IgA, IgM) before and after adsorption onto DNA bound to agarose-polylysine columns. Sixteen patients with systemic lupus erythematosus and 16 age- and sex-matched controls were studied. Precision was determined for high-value (in 10 patients) and low-value (in nine control...

Rate nephelometry and radial immunodiffusion compared for measuring serum prealbumin.

We compared a rate-nephelometric method and a radial immunodiffusion (RID) assay for measurement of prealbumin (transthyretin) in 55 samples of serum from healthy children. The mean prealbumin concentration as measured by the Beckman Auto ICS nephelometer was 188 mg/L (range 128-350); the mean by RID was 221 mg/L (range 125-419). This difference was statistically significant by Student's t-test...

DNA mobility shift assay coupled with SDS-PAGE for detection of DNA-binding proteins.

DNA mobility shift assay is widely performed to analyze sequence-specific DNA-binding proteins in cellular extracts (2), and the DNA-binding proteins can be identified by use of antibodies that induce further retardation of probes (supershift). Gel extracts from a shifted band contain not only DNA segments and DNA-binding proteins but also nonspecific proteins located at the same position in th...

channel estimation for mimo-ofdm systems

تخمین دقیق مشخصات کانال در سیستم های مخابراتی یک امر مهم محسوب می گردد. این امر به ویژه در کانال های بیسیم با ‏خاصیت فرکانس گزینی و زمان گزینی شدید، چالش بزرگی است. مقالات متعدد پر از روش های مبتکرانه ای برای طراحی و آنالیز ‏الگوریتم های تخمین کانال است که بیشتر آنها از روش های خاصی استفاده می کنند که یا دارای عملکرد خوب با پیچیدگی ‏محاسباتی بالا هستند و یا با عملکرد نه چندان خوب پیچیدگی پایینی...

Immunoglobulins with multiple binding functions. II. The use of nylon-polyserine whisker discs in screening myeloma immunoglobulins for binding activity.